alvetex scaffold 96 well plate reprocell (ReproCELL)
93
Structured Review
ReproCELL
alvetex scaffold 96 well plate reprocell
Alvetex Scaffold 96 Well Plate Reprocell, supplied by ReproCELL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alvetex+scaffold+96+well+plate+reprocell/Alvetex+Scaffold+96+well+plate+%C3%97+10/pm38866011-260-79-83
Average 93 stars, based on 1 article reviews
Alvetex Scaffold 96 Well Plate Reprocell, supplied by ReproCELL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alvetex+scaffold+96+well+plate+reprocell/Alvetex+Scaffold+96+well+plate+%C3%97+10/pm38866011-260-79-83
Average 93 stars, based on 1 article reviews
alvetex scaffold 96 well plate reprocell - by Bioz Stars,
2026-09
93/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Bidirectional activation of stem-like programs between metastatic cancer and alveolar type 2 cells within the niche. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# DN25-100MG 4’,6-diamidino-2-phenylindole (DAPI) Sigma-Aldrich Cat# D9542 Fetal bovine serum Labtech Cat# FB-1001/100-500 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15070063 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 HBSS, calcium/magnesium-free, no phenol red Thermo Fisher Scientific Cat# 14175095 DMEM Thermo Fisher Scientific Cat# 41965062 DMEM/F12 Thermo Fisher Scientific Cat# 11330032 Advanced DMEM/F12 Thermo Fisher Scientific Cat# 12634-010 Human EGF Thermo Fisher Scientific Cat# PHG0313 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Insulin solution human Sigma-Aldrich Cat# I9278 Insulin-Transferrin-Selenium (ITS) Thermo Fisher Scientific Cat# 41400045 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 Polybrene Sigma-Aldrich Cat# TR-1003-G Puromycin dihydrochloride APExBIO Cat# B7587 Antigenfix Diapath Cat# P0014 Zombie Aqua BioLegend Cat# 423101 ECL Chemiluminescence Detection Reagent Cytiva Cat# 10308449 D-luciferin PerkinElmer Cat# 122799 Critical commercial assays PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 SuperScript IV VILO with ezDNase Thermo Fisher Scientific Cat# 11766050 LS columns Miltenyi Biotec Cat# 130-042-401 RNeasy Mini Kit QIAGEN Cat# 74904 RNeasy Micro Kit QIAGEN Cat# 74004 Deposited data RNA-seq This study GSE240040 ATAC-seq This study GSE240040 Experimental models: Cell lines 4T1 ATCC Cat# CRL-2539 CT26 ATCC Cat# CRL-2638 4T07 The Francis Crick Institute N/A TS1 Gift from J. Joyce17 N/A B16F10 ATCC Cat# CRL-6475 MLE12 ATCC Cat# CRL-2110 MLg ATCC Cat# CCL-206 Experimental models: Organisms/strains Mouse: FVB/n The Francis Crick Institute Jax Strain # 001800 Mouse: BALB/c The Francis Crick Institute Jax Strain # 000651 Mouse: Sftpc-CreERT2;R26REYFP C57BL/6 Gift from J-H. Lee N/A Mouse: Actin-GFP FVB/n Gift from J. Huelsken N/A Mouse: tdTomato C57BL/6 The Francis Crick Institute N/A Mouse: MMTV-PyMT FVB/n The Francis Crick Institute Jax Strain # 022974 Mouse: MMTV-PyMT C57BL/6 The Francis Crick Institute N/A Mouse: Actin-GFP MMTV-PyMT FVB/n The Francis Crick Institute N/A Mouse: TSLP KO FVB/n The Francis Crick Institute N/A (Continued on next page) e2 Developmental Cell 59, 1–16.e1–e8, September 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR This paper N/A Recombinant DNA pLKO.1PURO non-mammalian targeting Sigma-Aldrich Clone ID: SHC002 pLKO.1PURO shSox9 #1 Sigma-Aldrich Clone ID: TRCN0000086165 pLKO.1PURO shSox9 #2 Sigma-Aldrich Clone ID: TRCN0000086167 Software and algorithms FlowJo software v.10.9.0 BD Life Sciences https://www.flowjo.com GraphPad Prism GraphPad https://www.graphpad.com Fiji/ImageJ Open source41 https://imagej.nih.gov/ij/ BioRender BioRender https://www.biorender.com StrataQuest TissueGnotics https://tissuegnostics.com/products/ contextual-image-analysis/strataquest Other 24-well transwell polyester membrane 0.4 mm Corning Cat# 3470; 24-well transwell polyester membrane 0.4 mm Greiner Cat# 662641 Recombinant:Article Title: Bidirectional activation of stem-like programs between metastatic cancer and alveolar type 2 cells within the niche. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# DN25-100MG 4’,6-diamidino-2-phenylindole (DAPI) Sigma-Aldrich Cat# D9542 Fetal bovine serum Labtech Cat# FB-1001/100-500 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15070063 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 HBSS, calcium/magnesium-free, no phenol red Thermo Fisher Scientific Cat# 14175095 DMEM Thermo Fisher Scientific Cat# 41965062 DMEM/F12 Thermo Fisher Scientific Cat# 11330032 Advanced DMEM/F12 Thermo Fisher Scientific Cat# 12634-010 Human EGF Thermo Fisher Scientific Cat# PHG0313 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Insulin solution human Sigma-Aldrich Cat# I9278 Insulin-Transferrin-Selenium (ITS) Thermo Fisher Scientific Cat# 41400045 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 Polybrene Sigma-Aldrich Cat# TR-1003-G Puromycin dihydrochloride APExBIO Cat# B7587 Antigenfix Diapath Cat# P0014 Zombie Aqua BioLegend Cat# 423101 ECL Chemiluminescence Detection Reagent Cytiva Cat# 10308449 D-luciferin PerkinElmer Cat# 122799 Critical commercial assays PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 SuperScript IV VILO with ezDNase Thermo Fisher Scientific Cat# 11766050 LS columns Miltenyi Biotec Cat# 130-042-401 RNeasy Mini Kit QIAGEN Cat# 74904 RNeasy Micro Kit QIAGEN Cat# 74004 Deposited data RNA-seq This study GSE240040 ATAC-seq This study GSE240040 Experimental models: Cell lines 4T1 ATCC Cat# CRL-2539 CT26 ATCC Cat# CRL-2638 4T07 The Francis Crick Institute N/A TS1 Gift from J. Joyce17 N/A B16F10 ATCC Cat# CRL-6475 MLE12 ATCC Cat# CRL-2110 MLg ATCC Cat# CCL-206 Experimental models: Organisms/strains Mouse: FVB/n The Francis Crick Institute Jax Strain # 001800 Mouse: BALB/c The Francis Crick Institute Jax Strain # 000651 Mouse: Sftpc-CreERT2;R26REYFP C57BL/6 Gift from J-H. Lee N/A Mouse: Actin-GFP FVB/n Gift from J. Huelsken N/A Mouse: tdTomato C57BL/6 The Francis Crick Institute N/A Mouse: MMTV-PyMT FVB/n The Francis Crick Institute Jax Strain # 022974 Mouse: MMTV-PyMT C57BL/6 The Francis Crick Institute N/A Mouse: Actin-GFP MMTV-PyMT FVB/n The Francis Crick Institute N/A Mouse: TSLP KO FVB/n The Francis Crick Institute N/A (Continued on next page) e2 Developmental Cell 59, 1–16.e1–e8, September 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR This paper N/A Recombinant DNA pLKO.1PURO non-mammalian targeting Sigma-Aldrich Clone ID: SHC002 pLKO.1PURO shSox9 #1 Sigma-Aldrich Clone ID: TRCN0000086165 pLKO.1PURO shSox9 #2 Sigma-Aldrich Clone ID: TRCN0000086167 Software and algorithms FlowJo software v.10.9.0 BD Life Sciences https://www.flowjo.com GraphPad Prism GraphPad https://www.graphpad.com Fiji/ImageJ Open source41 https://imagej.nih.gov/ij/ BioRender BioRender https://www.biorender.com StrataQuest TissueGnotics https://tissuegnostics.com/products/ contextual-image-analysis/strataquest Other 24-well transwell polyester membrane 0.4 mm Corning Cat# 3470; 24-well transwell polyester membrane 0.4 mm Greiner Cat# 662641 Software:Article Title: Bidirectional activation of stem-like programs between metastatic cancer and alveolar type 2 cells within the niche. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# DN25-100MG 4’,6-diamidino-2-phenylindole (DAPI) Sigma-Aldrich Cat# D9542 Fetal bovine serum Labtech Cat# FB-1001/100-500 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15070063 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 HBSS, calcium/magnesium-free, no phenol red Thermo Fisher Scientific Cat# 14175095 DMEM Thermo Fisher Scientific Cat# 41965062 DMEM/F12 Thermo Fisher Scientific Cat# 11330032 Advanced DMEM/F12 Thermo Fisher Scientific Cat# 12634-010 Human EGF Thermo Fisher Scientific Cat# PHG0313 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Insulin solution human Sigma-Aldrich Cat# I9278 Insulin-Transferrin-Selenium (ITS) Thermo Fisher Scientific Cat# 41400045 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 Polybrene Sigma-Aldrich Cat# TR-1003-G Puromycin dihydrochloride APExBIO Cat# B7587 Antigenfix Diapath Cat# P0014 Zombie Aqua BioLegend Cat# 423101 ECL Chemiluminescence Detection Reagent Cytiva Cat# 10308449 D-luciferin PerkinElmer Cat# 122799 Critical commercial assays PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 SuperScript IV VILO with ezDNase Thermo Fisher Scientific Cat# 11766050 LS columns Miltenyi Biotec Cat# 130-042-401 RNeasy Mini Kit QIAGEN Cat# 74904 RNeasy Micro Kit QIAGEN Cat# 74004 Deposited data RNA-seq This study GSE240040 ATAC-seq This study GSE240040 Experimental models: Cell lines 4T1 ATCC Cat# CRL-2539 CT26 ATCC Cat# CRL-2638 4T07 The Francis Crick Institute N/A TS1 Gift from J. Joyce17 N/A B16F10 ATCC Cat# CRL-6475 MLE12 ATCC Cat# CRL-2110 MLg ATCC Cat# CCL-206 Experimental models: Organisms/strains Mouse: FVB/n The Francis Crick Institute Jax Strain # 001800 Mouse: BALB/c The Francis Crick Institute Jax Strain # 000651 Mouse: Sftpc-CreERT2;R26REYFP C57BL/6 Gift from J-H. Lee N/A Mouse: Actin-GFP FVB/n Gift from J. Huelsken N/A Mouse: tdTomato C57BL/6 The Francis Crick Institute N/A Mouse: MMTV-PyMT FVB/n The Francis Crick Institute Jax Strain # 022974 Mouse: MMTV-PyMT C57BL/6 The Francis Crick Institute N/A Mouse: Actin-GFP MMTV-PyMT FVB/n The Francis Crick Institute N/A Mouse: TSLP KO FVB/n The Francis Crick Institute N/A (Continued on next page) e2 Developmental Cell 59, 1–16.e1–e8, September 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR This paper N/A Recombinant DNA pLKO.1PURO non-mammalian targeting Sigma-Aldrich Clone ID: SHC002 pLKO.1PURO shSox9 #1 Sigma-Aldrich Clone ID: TRCN0000086165 pLKO.1PURO shSox9 #2 Sigma-Aldrich Clone ID: TRCN0000086167 Software and algorithms FlowJo software v.10.9.0 BD Life Sciences https://www.flowjo.com GraphPad Prism GraphPad https://www.graphpad.com Fiji/ImageJ Open source41 https://imagej.nih.gov/ij/ BioRender BioRender https://www.biorender.com StrataQuest TissueGnotics https://tissuegnostics.com/products/ contextual-image-analysis/strataquest Other 24-well transwell polyester membrane 0.4 mm Corning Cat# 3470; 24-well transwell polyester membrane 0.4 mm Greiner Cat# 662641 Membrane:Article Title: Bidirectional activation of stem-like programs between metastatic cancer and alveolar type 2 cells within the niche. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deoxyribonuclease I from bovine pancreas Sigma-Aldrich Cat# DN25-100MG 4’,6-diamidino-2-phenylindole (DAPI) Sigma-Aldrich Cat# D9542 Fetal bovine serum Labtech Cat# FB-1001/100-500 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15070063 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 HBSS, calcium/magnesium-free, no phenol red Thermo Fisher Scientific Cat# 14175095 DMEM Thermo Fisher Scientific Cat# 41965062 DMEM/F12 Thermo Fisher Scientific Cat# 11330032 Advanced DMEM/F12 Thermo Fisher Scientific Cat# 12634-010 Human EGF Thermo Fisher Scientific Cat# PHG0313 B-27 Supplement Thermo Fisher Scientific Cat# 17504044 Insulin solution human Sigma-Aldrich Cat# I9278 Insulin-Transferrin-Selenium (ITS) Thermo Fisher Scientific Cat# 41400045 GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 Polybrene Sigma-Aldrich Cat# TR-1003-G Puromycin dihydrochloride APExBIO Cat# B7587 Antigenfix Diapath Cat# P0014 Zombie Aqua BioLegend Cat# 423101 ECL Chemiluminescence Detection Reagent Cytiva Cat# 10308449 D-luciferin PerkinElmer Cat# 122799 Critical commercial assays PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 SuperScript IV VILO with ezDNase Thermo Fisher Scientific Cat# 11766050 LS columns Miltenyi Biotec Cat# 130-042-401 RNeasy Mini Kit QIAGEN Cat# 74904 RNeasy Micro Kit QIAGEN Cat# 74004 Deposited data RNA-seq This study GSE240040 ATAC-seq This study GSE240040 Experimental models: Cell lines 4T1 ATCC Cat# CRL-2539 CT26 ATCC Cat# CRL-2638 4T07 The Francis Crick Institute N/A TS1 Gift from J. Joyce17 N/A B16F10 ATCC Cat# CRL-6475 MLE12 ATCC Cat# CRL-2110 MLg ATCC Cat# CCL-206 Experimental models: Organisms/strains Mouse: FVB/n The Francis Crick Institute Jax Strain # 001800 Mouse: BALB/c The Francis Crick Institute Jax Strain # 000651 Mouse: Sftpc-CreERT2;R26REYFP C57BL/6 Gift from J-H. Lee N/A Mouse: Actin-GFP FVB/n Gift from J. Huelsken N/A Mouse: tdTomato C57BL/6 The Francis Crick Institute N/A Mouse: MMTV-PyMT FVB/n The Francis Crick Institute Jax Strain # 022974 Mouse: MMTV-PyMT C57BL/6 The Francis Crick Institute N/A Mouse: Actin-GFP MMTV-PyMT FVB/n The Francis Crick Institute N/A Mouse: TSLP KO FVB/n The Francis Crick Institute N/A (Continued on next page) e2 Developmental Cell 59, 1–16.e1–e8, September 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides Primers for qPCR This paper N/A Recombinant DNA pLKO.1PURO non-mammalian targeting Sigma-Aldrich Clone ID: SHC002 pLKO.1PURO shSox9 #1 Sigma-Aldrich Clone ID: TRCN0000086165 pLKO.1PURO shSox9 #2 Sigma-Aldrich Clone ID: TRCN0000086167 Software and algorithms FlowJo software v.10.9.0 BD Life Sciences https://www.flowjo.com GraphPad Prism GraphPad https://www.graphpad.com Fiji/ImageJ Open source41 https://imagej.nih.gov/ij/ BioRender BioRender https://www.biorender.com StrataQuest TissueGnotics https://tissuegnostics.com/products/ contextual-image-analysis/strataquest Other 24-well transwell polyester membrane 0.4 mm Corning Cat# 3470; 24-well transwell polyester membrane 0.4 mm Greiner Cat# 662641 |